Name:

Prof. Antonio Llombart-Bosch

Centre:

Dept. Pathology. Medical School

Hospital Clinico Universitario

Av. Blasco Ibañez 16

Valencia 46010 - Spain

Tel. 34.963864146     Fax: 34.963864173

E-mail: antonio.llombart@uv.es

Professor of Anatomic Pathology. Head of Department of Pathology (Hospital Clinico Universitario). He has been involved since 1970 in the study of these tumors by morphological and biological means. Principal investigator in several national and international grants.

The Department of Pathology at the Medical School of the University of Valencia is connected with the Hospital Clinico Universitario, a 600 bed hospital and tumour reference center for the community of Valencia. The Department is a consultant centre for children’s tumours not only in the geographical area of Valencia (4.8 millions habitants) but for all Spain. Bone and soft tissue tumours are also collected from the Valencia Cancer Institute (IVO) in such a way that a large number of bone and soft tissue tumours are diagnosed and collected for second opinion in our Department.

Since 1970, we are mainly specialized in the study of the morphological and biological characteristics of ESFT and we have produced a number of major contributions in this field. Several grants have been obtained supporting this research, and joint projects with U.S. cancer centres have been carried out in this context (NIH, National Cancer Institute, Bethesda MD and Children’s Cancer Hospital, Los Angeles, CA). Several of the contributors to the present project (S. Navarro, R. Noguera), have had training experience in these centres of the US, with grants related to the study of Ewing’s sarcoma.

The principal Investigator of this project (Prof. A. Llombart-Bosch) has gathered extensive experience in the categorization of Ewing’s sarcoma and their differential diagnostic criteria from other small round cell tumors. Material from numerous major cancer centres of Europe has been reviewed (Institut Gustave Roussy, Villejuif; Russia Cancer Centre, Moscow; and Orthopedic Institute, Kiev). Material from numerous major cancer centers of Europe has been reviewed (Institut Gustave Roussy, Villejuif, All Russia Cancer Cenrter, Moscow, and Orthopedic Institute, Kiev) plus a large number of cases are available for research. A systematic procedure for the study of ESFT has been set up in the laboratory, including routine paraffin-embedded material and immunohistochemical analyses, a tissue bank with frozen tumours, and a collection of cell cultures characterized for cytogenetics, ultrastructure and molecular biology. DNA and RNA extracted from frozen tissue samples are also available in our tumour bank. Tissue culture, FISH, electron microscopy and cytogenetics are systematically performed in all these tumors following an established protocol, including molecular biology of gene rearrangements. A molecular biology laboratory complements these studies within our department of pathology.

Personnel involved in studies related to PROTHET's:

Amando Peydro-Olaya MD, PhD. Professor of Histology. Chief of the Electron Microscopy Laboratory. He is a well-known expert in electron microscopy, and has participated in the study of Ewing’s sarcoma since the initial studies.

Antonio Pellin-Perez PhD. Professor of Cellular Biology. Chief of the Molecular Biology Laboratory. He is involved in the study of genetic rearrangements in bone and soft tissue tumours.

Samuel Navarro MD, PhD. Professor of Anatomic Pathology. Chief of the Laboratory of Immunohistochemistry at the Hospital Clinico Universitario. He has extensive experience in the diagnosis and characterization of ESFT as well as neuroblastoma.

Carmen Carda MD, PhD. Professor of Histology. She is in charge of the nude mice studies and tumor xenografts, with an experience of over 15 years in this field.

Rosa Noguera MD PhD. Professor of Histology. She is an expert in tissue culture, cytogenetics and FISH analysis of children’s tumours and particularly she is in charge of the study of small round cell tumours (ESFT and neuroblastoma).

JA Lopez Guerrero, PhD. Research fellow at the Department of Pathology. He participates in the molecular biology study of ESFT (fresh tissue samples, cell cultures and paraffin-embedded material).  

Recent Publications relevant to the project:

Peydró-Olaya A, Llombart-Bosch A, Carda-Batalla C, López-Guerrero JA. Electron microscopy and other ancillary techniques in the diagnosis of small round cell tumors. Sem Diagn Pathol (in press, 2003).

Navarro S, Noguera R, Pellin A, Lopez-Guerrero JA, Rosello-Sastre E, Cremades A, Llombart-Bosch A. Atypical pleomorphic extraosseous ewing tumor/peripheral primitive neuroectodermal tumor with unusual phenotypic/genotypic profile. Diagn Mol Pathol 11:9-15, 2002.

Lopez-Guerrero JA, Pellin A, Noguera R, Carda C, Llombart-Bosch A. Molecular analysis of the 9p21 locus and p53 genes in Ewing family tumors. Lab Invest 81:803-814, 2001.

Noguera R, Pellin A, Navarro S, Carda C, Llombart-Bosch A. Translocation (10;11;22)(p14;q24;q12) characterized by fluorescence in situ hybridization in a case of Ewing’s tumor. Diagn Mol Pathol 10:2-8, 2001.

Llombart-Bosch A, Navarro S. Immunohistochemical detection of EWS and FLI1 proteins in Ewing’s sarcoma and primitive neuroectodermal tumors. Comparative analysis with CD-99 (MIC2) expression. Appl Immunohistochem Mol Morphol 9:255-260, 2001.